Method for Imaging Human Sperm Cells (In collaboration with Prof Mark Wilson Biological Science University of Wollongong)

 

1 Whole semen (3ml) dilute with Phosphate Buffered Saline (PBS) to 15ml. mixed & allowed to stand for 1-2 min to allow large debris to settle.

2 Supernatant gently removed & then centrifuged (1600 rpm, 300xg for 5min). Supernatant discarded. Pellet re-susupended in PBS to 15 ml & centrifuged (as above) again.

3 Cell pellet washed once more as above & then re-suspended in 1ml of glutaraldehyde in PBS. incubated on ice for 30min (to chemically fix the cells)

4 Cells then applied to a glass slide & examined using a Leica DMIRBE inverted light microscope using phase contrast optics. 100x oil immersion objective.

5 Images were captured using a Leica DC500 Digital Camera.

Health and sperm count (pdf link)